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ME3 antibody

Anti-ME3 antibody used in Western Blot (WB). GTX130689

Cat. No. GTX130689

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all ME3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

mouse hippocampus

Predict Reactivity

Human, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ME3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886321

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

malic enzyme 3 , NADP-ME

Cellular Localization

Mitochondrion matrix

Background

Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ME3 antibody used in Western Blot (WB). GTX130689

GTX130689 WB Image

ME3 antibody detects ME3 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with ME3 antibody (GTX130689) at a dilution of 1:500.

REFERENCE

Application Reference

White K et al. Sci Rep 2018; 8 (1) : 5039 Loss of IDH2 Accelerates Age-related Hearing Loss in Male Mice.
Application : WB
Reactivity : Mouse
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169