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MECP2 (phospho Ser80) antibody

Anti-MECP2 (phospho Ser80) antibody used in Western Blot (WB). GTX82658

Cat. No. GTX82658

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all MECP2 products

APPLICATION

Application Note

Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM HEPES, 150mM NaCl, 0.01% BSA, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser80 conjugated to KLH

Purification

Serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methyl-CpG binding protein 2 , AUTSX3 , MRX16 , MRX79 , MRXS13 , MRXSL , PPMX , RS , RTS , RTT

Cellular Localization

Nucleus

Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-MECP2 (phospho Ser80) antibody used in Western Blot (WB). GTX82658

GTX82658 WB Image

Western blot of neonatal rat brain showing specific immunolabeling of the ~75k MeCP2 protein phosphorylated at Ser 80 using MeCP2 (phospho Ser80) antibody (GTX82658).

REFERENCE

Application Reference

Lee LC et al. Mol Brain 2021; 14 (1) : 152 Association of CaMK2A and MeCP2 signaling pathways with cognitive ability in adolescents.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Package List Price ($)
$ 439