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MED1 antibody

Anti-MED1 antibody used in Western Blot (WB). GTX66658
Anti-MED1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66658

Cat. No. GTX66658

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all MED1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

168 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 664-720 of Human TRAP220.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mediator complex subunit 1 , CRSP1 , CRSP200 , DRIP205 , DRIP230 , PBP , PPARBP , PPARGBP , RB18A , TRAP220 , TRIP2

Cellular Localization

Nucleus

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MED1 antibody used in Western Blot (WB). GTX66658

GTX66658 WB Image

WB analysis of various samples using GTX66658 MED1 antibody.
Lane1 : A549 whole cell lysate(40ug)
Lane2 : HEK293T whole cell lysate(40ug)
Lane3 : PC12 whole cell lysate(40ug)
Lane4 : CT26 whole cell lysate(40ug)
Dilution : 1:500

Anti-MED1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66658

GTX66658 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX66658 MED1 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399