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MED14 antibody

Anti-MED14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87124
Anti-MED14 antibody used in IHC (Paraffin sections) (IHC-P). GTX87124

Cat. No. GTX87124

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human, Monkey
Package
100 μg ($399)
See all MED14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MED14 (701-750).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CRSP150 , CRSP2 , CSRP , CXorf4 , DRIP150 , EXLM1 , MED14 , RGR1 , TRAP170 , mediator complex subunit 14

Cellular Localization

Nucleus

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MED14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87124

GTX87124 ICC/IF Image

ICC/IF analysis of COS7 cells using GTX87124 MED14 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MED14 antibody used in IHC (Paraffin sections) (IHC-P). GTX87124

GTX87124 IHC-P Image

IHC-P analysis of human brain tissue using GTX87124 MED14 antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399