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MEK1 + MEK2 (phospho Ser222) antibody

Orthogonal
Anti-MEK1 + MEK2 (phospho Ser222) antibody used in Western Blot (WB). GTX24750
Anti-MEK1 + MEK2 (phospho Ser222) antibody used in Western Blot (WB). GTX24750
Anti-MEK1 + MEK2 (phospho Ser222) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24750

Cat. No. GTX24750

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
50 μl ($399)
See all MEK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Immunogen is a sequence surrounding the phosphorylated Ser222 of MEK1/2.

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_380407

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Microtubule-associated protein kinase kinase 1 + 2 (MEK 1 + 2 or MAPKK 1+ 2) are members of a tyrosine/threonine protein kinase family that activate the ERK 1 + 2/MAPK enzymes by phosphorylating both residues within the threonine - glutamic acid - tyrosine (TEY) motif in the activation loop. MEK 1 + 2 are also activated by dual-phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs.

Research Area

DATA IMAGES

Anti-MEK1 + MEK2 (phospho Ser222) antibody used in Western Blot (WB). GTX24750

GTX24750 WB Image

Orthogonal

WB analysis of whole cell extracts (30 ug lysate) of A549 (Lane 1), treated with EGF (200 ng/ml for 10 min) (Lane 2), and treated with Afatinib (0.5 uM for 16 h) followed by EGF (200 ng/ml for 10 min) using GTX24750 MEK1 + MEK2 (phospho Ser222) antibody.
Dilution : 1:1000

Anti-MEK1 + MEK2 (phospho Ser222) antibody used in Western Blot (WB). GTX24750

GTX24750 WB Image

Orthogonal

WB (peptide competition) analysis of NIH3T3 cells treated with 50 ng/mL PDGF for 15 minutes (Lane 2-5) using GTX24750 MEK1 + MEK2 (phospho Ser222) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Anti-MEK1 + MEK2 (phospho Ser222) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24750

GTX24750 ICC/IF Image

ICC/IF analysis of mouse embryonic fibroblasts (MEFs) using GTX24750 MEK1 + MEK2 (phospho Ser222) antibody. The cell is actively dividing. Blue represents chromosomes in anaphase of mitotic cell division. Green shows mitotic spindle expressing phosphorylated MEK1, 2.

REFERENCE

Application Reference

Lin JT et al. Journal of Functional Foods 2018; 47 : 343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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Package List Price ($)
$ 399