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MEK1 antibody [1F5]

KO/KD Overexpression
Anti-MEK1 antibody [1F5] used in Western Blot (WB). GTX84167
Anti-MEK1 antibody [1F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84167
Anti-MEK1 antibody [1F5] used in Western Blot (WB). GTX84167

Cat. No. GTX84167

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F5

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($519)
See all MEK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:1000
ICC/IF 1:100
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human MAP2K1 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 1 , CFC3 , MAPKK1 , MEK1 , MKK1 , PRKMK1

Cellular Localization

Cytoplasm,Nucleus,Membrane

Background

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Database

Research Area

DATA IMAGES

Anti-MEK1 antibody [1F5] used in Western Blot (WB). GTX84167

GTX84167 WB Image

KO/KD

WB analysis of wild type and MEK1 knock out HeLa cell lysate(10μg per lane) using GTX84167 MEK1 antibody [1F5].
Dilution : 1:500

Anti-MEK1 antibody [1F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84167

GTX84167 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with MEK1 plasmid using GTX84167 MEK1 antibody [1F5].

Anti-MEK1 antibody [1F5] used in Western Blot (WB). GTX84167

GTX84167 WB Image

Overexpression

WB analysis of HEK293T cells transfected with MEK1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84167 MEK1 antibody [1F5].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519