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METTL1 antibody [HL2041]

Recombinant antibody Orthogonal
Anti-METTL1 antibody [HL2041] used in Western Blot (WB). GTX637942
Anti-METTL1 antibody [HL2041] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637942

Cat. No. GTX637942

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2041

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all METTL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human METTL1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methyltransferase like 1 , C12orf1 , TRM8 , TRMT8 , YDL201w

Cellular Localization

Nucleus

Background

This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-METTL1 antibody [HL2041] used in Western Blot (WB). GTX637942

GTX637942 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with METTL1 antibody [HL2041] (GTX637942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-METTL1 antibody [HL2041] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637942

GTX637942 ICC/IF Image

METTL1 antibody [HL2041] detects METTL1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: RMS-13 cells were fixed in ice-cold MeOH for 5 min.
Green: METTL1 stained by METTL1 antibody [HL2041] (GTX637942) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations