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METTL14 antibody [HL1816]

Recombinant antibody KO/KD
Anti-METTL14 antibody [HL1816] used in Western Blot (WB). GTX637541
Anti-METTL14 antibody [HL1816] used in IHC (Paraffin sections) (IHC-P). GTX637541
Anti-METTL14 antibody [HL1816] used in IHC (Paraffin sections) (IHC-P). GTX637541
Anti-METTL14 antibody [HL1816] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637541
Anti-METTL14 antibody [HL1816] used in Western Blot (WB). GTX637541
Anti-METTL14 antibody [HL1816] used in Western Blot (WB). GTX637541
Anti-METTL14 antibody [HL1816] used in Western Blot (WB). GTX637541
Anti-METTL14 antibody [HL1816] used in Western Blot (WB). GTX637541
Anti-METTL14 antibody [HL1816] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637541

Cat. No. GTX637541

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1816

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all METTL14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human METTL14.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methyltransferase like 14 , hMETTL14

Cellular Localization

Nucleus

Database

DATA IMAGES

Anti-METTL14 antibody [HL1816] used in Western Blot (WB). GTX637541

GTX637541 WB Image

KO/KD

Untransfected (–) and transfected (+) A549 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (GTX637541) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-METTL14 antibody [HL1816] used in IHC (Paraffin sections) (IHC-P). GTX637541

GTX637541 IHC-P Image

METTL14 antibody [HL1816] detects METTL14 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
METTL14 stained by METTL14 antibody [HL1816] (GTX637541) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-METTL14 antibody [HL1816] used in IHC (Paraffin sections) (IHC-P). GTX637541

GTX637541 IHC-P Image

METTL14 antibody [HL1816] detects METTL14 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
METTL14 stained by METTL14 antibody [HL1816] (GTX637541) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-METTL14 antibody [HL1816] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637541

GTX637541 ICC/IF Image

METTL14 antibody [HL1816] detects METTL14 protein by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: METTL14 stained by METTL14 antibody [HL1816] (GTX637541) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-METTL14 antibody [HL1816] used in Western Blot (WB). GTX637541

GTX637541 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (GTX637541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-METTL14 antibody [HL1816] used in Western Blot (WB). GTX637541

GTX637541 WB Image

Various mouse tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (GTX637541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-METTL14 antibody [HL1816] used in Western Blot (WB). GTX637541

GTX637541 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (GTX637541) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-METTL14 antibody [HL1816] used in Western Blot (WB). GTX637541

GTX637541 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (GTX637541) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-METTL14 antibody [HL1816] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637541

GTX637541 ICC/IF Image

METTL14 antibody [HL1816] detects METTL14 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: METTL14 stained by METTL14 antibody [HL1816] (GTX637541) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations