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METTL3 antibody

Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315
Anti-METTL3 antibody used in Immunoprecipitation (IP). GTX33315
Anti-METTL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33315
Anti-METTL3 antibody used in Western Blot (WB). GTX33315
Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315

Cat. No. GTX33315

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all METTL3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methyltransferase like 3 , IME4 , M6A , MT-A70 , Spo8 , hMETTL3

Cellular Localization

Nucleus,Nucleus speckle,Cytoplasm

Background

This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315

GTX33315 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33315 METTL3 antibody.
Blue : DAPI
Dilution : 1:200

Anti-METTL3 antibody used in Immunoprecipitation (IP). GTX33315

GTX33315 IP Image

IP analysis of 293T cell lysate using GTX33315 METTL3 antibody.
Antibody amount : 3μg / 150μg lysate
Dilution : 1:1000

Anti-METTL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33315

GTX33315 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX33315 METTL3 antibody.
Dilution : 1:100

Anti-METTL3 antibody used in Western Blot (WB). GTX33315

GTX33315 WB Image

WB analysis of various sample lysates using GTX33315 METTL3 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315

GTX33315 ICC/IF Image

ICC/IF analysis of U-2 OS cells using GTX33315 METTL3 antibody.
Blue : DAPI for nuclear staining

REFERENCE

Application Reference

Li K et al. J Cell Commun Signal 2023; 17 (3) : 897-914 METTL3-METTL14 complex induces necroptosis and inflammation of vascular smooth muscle cells via promoting N6 methyladenosine mRNA methylation of receptor-interacting protein 3 in abdominal aortic aneurysms.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399