APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2 μg/ml |
1-2 μg/ml |
1-2 μg/ml |
1-2 μg/million cells |
2-5 μg/ml (for coating) |
Assay dependent |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
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Not tested in other applications.
Observed MW
33-35 kDa. The observed M.W. is based on the following publication. PMID: 9931307
Product Note
It reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
200 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
major histocompatibility complex, class II, DP beta 1 , major histocompatibility complex, class II, DQ alpha 1 , major histocompatibility complex, class II, DR alpha , DPB1 , HLA-DP , HLA-DP1B , HLA-DPB , CELIAC1 , DQ-A1 , DQA1 , HLA-DQA , HLA-DRA1
Cellular Localization
Cell membrane; Single-pass type I membrane protein
Research Area
DATA IMAGES
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GTX02758 ICC/IF Image
ICC/IF analysis of PFA fixed Ramos cells using GTX02758 MHC Class II antibody [HLA-Pan/2967R]. Green : Primary antibody Red : nuclear counterstain
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GTX02758 WB Image
WB analysis of Ramos whole cell lysate using GTX02758 MHC Class II antibody [HLA-Pan/2967R].
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GTX02758 IHC-P Image
IHC-P analysis of human tonsil tissue section using GTX02758 MHC Class II antibody [HLA-Pan/2967R].
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GTX02758 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using HLA-Pan Rabbit Recombinant Monoclonal Antibody (HLA-Pan/2967R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD') above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD') between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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GTX02758 FACS Image
FACS analysis of Raji cells using GTX02758 MHC Class II antibody [HLA-Pan/2967R]. Blue : Primary antibody Red : Isotype control
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GTX02758 ICC/IF Image
ICC/IF analysis of PFA fixed Raji cells using GTX02758 MHC Class II antibody [HLA-Pan/2967R]. Green : Primary antibody Red : nuclear counterstain
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REFERENCE
There are currently no references for MHC Class II antibody [HLA-Pan/2967R] (GTX02758). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MHC Class II antibody [HLA-Pan/2967R] (GTX02758). Be the first to share your experience with this product.