MHC Class II antibody [rHLA-Pan/3475]

Cat. No. GTX02759

Host	Mouse
Clonality	Monoclonal
Clone Name	rHLA-Pan/3475
Isotype	IgG1
Application	ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human
Package	100 μg ($529)

See all MHC Class II products

Datasheet

View product citations for antibody GTX02759 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ICC/IF	1-2 μg/ml
IHC-P	1-2 μg/ml
FACS	1-2 μg/million cells
ELISA	1-5 μg/ml (for coating)
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes. ELISA For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Product Note

It reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Non-T, non-B human acute lymphoblastic leukemia REH6 cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class II, DP beta 1 , major histocompatibility complex, class II, DQ alpha 1 , major histocompatibility complex, class II, DR alpha , DPB1 , HLA-DP , HLA-DP1B , HLA-DPB , CELIAC1 , DQ-A1 , DQA1 , HLA-DQA , HLA-DRA1

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Research Area

DATA IMAGES

GTX02759 ICC/IF Image

ICC/IF analysis of Raji cells using GTX02759 MHC Class II antibody [rHLA-Pan/3475].
Green : Primary antibody
Red : nuclear counterstain

GTX02759 FACS Image

FACS analysis of Raji cells using GTX02759 MHC Class II antibody [rHLA-Pan/3475].
Blue : Primary antibody
Red : Isotype control

GTX02759 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX02759 MHC Class II antibody [rHLA-Pan/3475].

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 529

Qty

MHC Class II antibody [rHLA-Pan/3475]

Cat. No. GTX02759

Host

Clonality

Clone Name

Isotype

Application

Reactivity