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MIER3 antibody

Anti-MIER3 antibody used in IHC (Paraffin sections) (IHC-P). GTX121542
Anti-MIER3 antibody used in IHC (Paraffin sections) (IHC-P). GTX121542
Anti-MIER3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121542
Anti-MIER3 antibody used in Western Blot (WB). GTX121542
Anti-MIER3 antibody used in IHC (Paraffin sections) (IHC-P). GTX121542

Cat. No. GTX121542

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MIER3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MIER3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165203

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MIER family member 3

Cellular Localization

Nucleus

Background

Transcriptional repressor.

Database

Research Area

DATA IMAGES

Anti-MIER3 antibody used in IHC (Paraffin sections) (IHC-P). GTX121542

GTX121542 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Huh7 xenograft, using MIER3 antibody(GTX121542) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MIER3 antibody used in IHC (Paraffin sections) (IHC-P). GTX121542

GTX121542 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using MIER3 antibody(GTX121542) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MIER3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121542

GTX121542 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using MIER3(GTX121542) antibody at 1:500 dilution.

Anti-MIER3 antibody used in Western Blot (WB). GTX121542

GTX121542 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX121542 diluted at 1:1000

Anti-MIER3 antibody used in IHC (Paraffin sections) (IHC-P). GTX121542

GTX121542 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using MIER3 antibody(GTX121542) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Huang W et al. Exp Cell Res 2021; 406 (1) : 112722 MIER3 induces epithelial-mesenchymal transition and promotes breast cancer cell aggressiveness via forming a co-repressor complex with HDAC1/HDAC2/Snail.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169