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MLKL antibody

KO/KD
Anti-MLKL antibody used in Western Blot (WB). GTX107538
Anti-MLKL antibody used in Western Blot (WB). GTX107538

Cat. No. GTX107538

Reference 29
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MLKL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

THP-1

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MLKL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037439

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mixed lineage kinase domain like pseudokinase , hMLKL

Database

Research Area

DATA IMAGES

Anti-MLKL antibody used in Western Blot (WB). GTX107538

GTX107538 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MLKL antibody (GTX107538) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MLKL antibody used in Western Blot (WB). GTX107538

GTX107538 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MLKL antibody (GTX107538) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liu C et al. Nat Commun 2024; 15 (1) : 2083 Targeting P2Y(14)R protects against necroptosis of intestinal epithelial cells through PKA/CREB/RIPK1 axis in ulcerative colitis.
Liu S et al. Cell Death Differ 2024; 31 (1) : 40-52 MLKL polymerization-induced lysosomal membrane permeabilization promotes necroptosis.
Zhou H et al. Commun Biol 2023; 6 (1) : 805
Chen J et al. Front Oncol 2022; 12 : 907036 Cell Death Triggers Induce MLKL Cleavage in Multiple Myeloma Cells, Which may Promote Cell Death.
Application : IP
Reactivity : Human
Du J et al. Nat Commun 2021; 12 (1) : 7067 RIPK1 dephosphorylation and kinase activation by PPP1R3G/PP1γ promote apoptosis and necroptosis.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

MLKL antibody Cat. No. GTX107538

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-MLKL antibody used in Western Blot (WB). GTX107538
Date : Anonymous submitted on 27-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT-29 cells
Blocking : 5% Skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Image courtesy of Shota Sakaguchi from Kyoto University.
Submit a Review
Application Reference
Liu C et al. Nat Commun 2024; 15 (1):2083 Targeting P2Y(14)R protects against necroptosis of intestinal epithelial cells through PKA/CREB/RIPK1 axis in ulcerative colitis.
Liu S et al. Cell Death Differ 2024; 31 (1):40-52 MLKL polymerization-induced lysosomal membrane permeabilization promotes necroptosis.
Zhou H et al. Commun Biol 2023; 6 (1):805
Chen J et al. Front Oncol 2022; 12:907036 Cell Death Triggers Induce MLKL Cleavage in Multiple Myeloma Cells, Which may Promote Cell Death.
Application : IP
Reactivity : Human
Du J et al. Nat Commun 2021; 12 (1):7067 RIPK1 dephosphorylation and kinase activation by PPP1R3G/PP1γ promote apoptosis and necroptosis.
Application : WB
Reactivity : Human
Zu R et al. Cell Death Dis 2021; 12 (11):994 Quantitative analysis of phosphoproteome in necroptosis reveals a role of TRIM28 phosphorylation in promoting necroptosis-induced cytokine production.
Application : WB
Reactivity : Human
Palmer S et al. Mol Biol Evol 2021; (Epub) Evolutionary profile for (host and viral) MLKL indicates its activities as a battlefront for extensive counteradaptation.
Application : WB
Reactivity : Human
Tonnus W et al. Nat Commun 2021; 12 (1):4402 Dysfunction of the key ferroptosis-surveilling systems hypersensitizes mice to tubular necrosis during acute kidney injury.
Application : WB
Reactivity : Human
Johnston AN et al. Proc Natl Acad Sci U S A 2020; 117 (12):6521-6530 Necroptosis-blocking compound NBC1 targets heat shock protein 70 to inhibit MLKL polymerization and necroptosis.
Hanna-Addams S et al. Proc Natl Acad Sci U S A 2020; 117 (4):1962-1970 CK1α, CK1δ, and CK1ε are necrosome components which phosphorylate serine 227 of human RIPK3 to activate necroptosis.
Seo J et al. Cell Death Differ 2020; (Epub) Beclin 1 functions as a negative modulator of MLKL oligomerisation by integrating into the necrosome complex.
Application : WB
Reactivity : Human
Dai J et al. Cell Death Dis 2020; 11 (4):282 A necroptotic-independent function of MLKL in regulating endothelial cell adhesion molecule expression.
Application : IP
Reactivity : Human
Lin YC et al. Aging (Albany NY) 2020; 12 (7):5764-5780 CK1δ as a potential therapeutic target to treat bladder cancer.
Application : WB
Reactivity : Human
Shrestha A et al. mBio 2019; 10 (6) RIP1, RIP3, and MLKL Contribute to Cell Death Caused by Clostridium perfringens Enterotoxin.
Mishra SK et al. Biochim Biophys Acta Mol Cell Biol Lipids 2019; 1864 (2):158-167 Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells.
Application : WB
Reactivity : Human
Weber K et al. Commun Biol 2018; 1:6 Nuclear RIPK3 and MLKL contribute to cytosolic necrosome formation and necroptosis.
Lan F et al. Am J Respir Crit Care Med 2018; (Epub) Staphylococcus aureus Induces Mucosal Type 2 Immune Response via Epithelial Cell Derived Cytokines.
Application : WB
Reactivity : Human
Weber K et al. Communications Biology 2018; (Epub) Nuclear RIPK3 and MLKL contribute to cytosolic necrosome formation and necroptosis.
Application : WB
Reactivity : Human
Liu S et al. Proc Natl Acad Sci U S A 2017; 114 (36):E7450-E7459 MLKL forms disulfide bond-dependent amyloid-like polymers to induce necroptosis.
Martens S et al. Cell Death Dis 2017; 8 (6):e2904 Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury
Application : WB
Feldmann F et al. Oncotarget 2017; 8 (40):68208-68220 * inhibits therapeutic induction of necroptosis in acute leukemia cells.
Application : WB
Reactivity : Human
Reynoso E et al. J Biol Chem 2017; (Epub) Thioredoxin-1 actively maintains the pseudokinase MLKL in a reduced state to suppress disulfide bond-dependent MLKL polymer formation and necroptosis.
Application : WB
Reactivity : Human
Cekay MJ et al. Cancer Lett 2017; (Epub) smac mimetics and type ii * synergistically induce necroptosis in various cancer cell lines.
Application : WB
Reactivity : Human
Bhatti IA et al. Cancer Lett 2017; (Epub) identification of a synergistic combination of smac mimetic and * to trigger cell death in b-cell non-hodgkin lymphoma cells.
Dasari SK et al. Cell Death Differ 2017; (Epub) Signalome-wide RNAi screen identifies GBA1 as a positive mediator of autophagic cell death.
Application : WB
Reactivity : Human
Aredia F et al. BMC Cancer 2016; 16 (1):851 Molecular features of the cytotoxicity of an NHE inhibitor: Evidence of mitochondrial alterations, ROS overproduction and DNA damage.
Application : WB
Reactivity : Human
Safferthal C et al. Oncogene 2016; Therapeutic targeting of necroptosis by Smac mimetic bypasses apoptosis resistance in acute myeloid leukemia cells.
Application : WB
Reactivity : Human
Rohde K et al. Cell Death Differ 2016; (Epub) A Bak-dependent mitochondrial amplification step contributes to Smac mimetic/glucocorticoid-induced necroptosis.
Application : WB
Reactivity : Human
Yoon S et al. Cell Death Differ 2015; (Epub) Necroptosis is preceded by nuclear translocation of the signaling proteins that induce it.
Application : WB, IP
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169