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MMP10 antibody [N1C1]

Overexpression
Anti-MMP10 antibody [N1C1] used in Western Blot (WB). GTX113496
Anti-MMP10 antibody [N1C1] used in Western Blot (WB). GTX113496
Anti-MMP10 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113496

Cat. No. GTX113496

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MMP10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

human MMP10-transfected 293T , HepG2 conditioned medium

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169002

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 10 , SL-2 , STMY2

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MMP10 antibody [N1C1] used in Western Blot (WB). GTX113496

GTX113496 WB Image

Overexpression

MMP10 antibody detects MMP10 protein by western blot analysis. Non-transfected (-) and MMP10-transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP10 antibody (GTX113496) diluted at 1:5000.

Anti-MMP10 antibody [N1C1] used in Western Blot (WB). GTX113496

GTX113496 WB Image

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MMP10 antibody [N1C1] (GTX113496) diluted at 1:1000.

Anti-MMP10 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113496

GTX113496 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using MMP10(GTX113496) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Quilty F et al. Int J Mol Sci 2020; 21 (21) Impact of Deoxycholic Acid on Oesophageal Adenocarcinoma Invasion: Effect on Matrix Metalloproteinases.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169