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MOB1B antibody [1H2]

Anti-MOB1B antibody [1H2] used in Flow cytometry (FACS). GTX84105
Anti-MOB1B antibody [1H2] used in IHC (Paraffin sections) (IHC-P). GTX84105
Anti-MOB1B antibody [1H2] used in Western Blot (WB). GTX84105
Anti-MOB1B antibody [1H2] used in IHC (Paraffin sections) (IHC-P). GTX84105
Anti-MOB1B antibody [1H2] used in IHC (Paraffin sections) (IHC-P). GTX84105
Anti-MOB1B antibody [1H2] used in Flow cytometry (FACS). GTX84105

Cat. No. GTX84105

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1H2

Isotype

 IgG2a

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all MOB1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human MOBKL1A(NP_775739) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MOB kinase activator 1B , MATS2 , MOB4A , MOBKL1A

Cellular Localization

Cytoplasm,Nucleus

Background

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.

Database

Research Area

DATA IMAGES

Anti-MOB1B antibody [1H2] used in Flow cytometry (FACS). GTX84105

GTX84105 FACS Image

FACS analysis of HeLa cells using GTX84105 MOB1B antibody [1H2].
Red : Primary antibody
Blue : Negative control antibody

Anti-MOB1B antibody [1H2] used in IHC (Paraffin sections) (IHC-P). GTX84105

GTX84105 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84105 MOB1B antibody [1H2].
Dilution : 1:150

Anti-MOB1B antibody [1H2] used in Western Blot (WB). GTX84105

GTX84105 WB Image

WB analysis of HEK293T cells transfected with MOB1B plasmid (Right) or empty vector (Left) for 48 hrs using GTX84105 MOB1B antibody [1H2].
Loading : 5 ug per lane

Anti-MOB1B antibody [1H2] used in IHC (Paraffin sections) (IHC-P). GTX84105

GTX84105 IHC-P Image

IHC-P analysis of human liver tissue using GTX84105 MOB1B antibody [1H2].
Dilution : 1:150

Anti-MOB1B antibody [1H2] used in IHC (Paraffin sections) (IHC-P). GTX84105

GTX84105 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84105 MOB1B antibody [1H2].
Dilution : 1:150

Anti-MOB1B antibody [1H2] used in Flow cytometry (FACS). GTX84105

GTX84105 FACS Image

FACS analysis of Jurkat cells using GTX84105 MOB1B antibody [1H2].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519