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MP1 antibody [N1C3]

Anti-MP1 antibody [N1C3] used in Western Blot (WB). GTX111032
Anti-MP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX111032
Anti-MP1 antibody [N1C3] used in Western Blot (WB). GTX111032
Anti-MP1 antibody [N1C3] used in Western Blot (WB). GTX111032

Cat. No. GTX111032

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

293T , Rat2 , Neuro2A , Raw264.7 , C2C12

Predict Reactivity

Zebrafish, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human MP1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950858

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 , MAP2K1IP1 , MAPBP , MAPKSP1 , MP1 , PRO0633 , Ragulator3

Background

This gene encodes a scaffold protein that functions in the extracellular signal-regulated kinase (ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2. Studies of the orthologous gene in mouse indicate that it regulates late endosomal traffic and cell proliferation. Multiple transcript variants are expressed by this gene, but only one variant is thought to express a protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MP1 antibody [N1C3] used in Western Blot (WB). GTX111032

GTX111032 WB Image

MP1 antibody detects MP1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg Raw264.7 whole cell lysate/extract
C. 30 μg C2C12 whole cell lysate/extract
15 % SDS-PAGE
MP1 antibody (GTX111032) dilution: 1:1000

Anti-MP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX111032

GTX111032 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MP1(GTX111032) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MP1 antibody [N1C3] used in Western Blot (WB). GTX111032

GTX111032 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
15% SDS PAGE
GTX111032 diluted at 1:1000

Anti-MP1 antibody [N1C3] used in Western Blot (WB). GTX111032

GTX111032 WB Image

MP1 antibody detects MP1 protein by Western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
15 % SDS-PAGE
MP1 antibody (GTX111032) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169