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MPP5 antibody, N-term

Anti-MPP5 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89702
Anti-MPP5 antibody, N-term used in Western Blot (WB). GTX89702
Anti-MPP5 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89702

Cat. No. GTX89702

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all MPP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89702-PEP).

Predict Reactivity

Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence NKASPPFPLISNAQ-C, from the N Terminus of the protein sequence according to NP_071919.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

membrane palmitoylated protein 5 , PALS1

Background

This gene encodes a member of the p55-like subfamily of the membrane-associated guanylate kinase (MAGUK) gene superfamily. The encoded protein participates in the polarization of differentiating cells, has been shown to regulate myelinating Schwann cells (PMID: 20237282), and is one of the components of the Crumbs complex in the retina. Mice which express lower levels of the orthologous protein have retinal degeneration and impaired vision (PMID: 22114289). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-MPP5 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89702

GTX89702 IHC-P Image

IHC-P analysis of paraffin embedded human small intestine using GTX89702 MPP5 antibody, N-term.
Antigen retrieval : Steamed antigen retrieval with citrate buffer pH 6
Dilution : 5μg/ml

Anti-MPP5 antibody, N-term used in Western Blot (WB). GTX89702

GTX89702 WB Image

WB analysis of human brain lysate using GTX89702 MPP5 antibody, N-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-MPP5 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89702

GTX89702 IHC-P Image

IHC-P analysis of paraffin embedded human kidney using GTX89702 MPP5 antibody, N-term.
Antigen retrieval : Steamed antigen retrieval with citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399