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MPST antibody [C2C3], C-term

KO/KD Orthogonal
Anti-MPST antibody [C2C3], C-term used in Western Blot (WB). GTX108274
Anti-MPST antibody [C2C3], C-term used in Western Blot (WB). GTX108274
Anti-MPST antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108274
Anti-MPST antibody [C2C3], C-term used in Western Blot (WB). GTX108274
Anti-MPST antibody [C2C3], C-term used in Western Blot (WB). GTX108274

Cat. No. GTX108274

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MPST products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

mouse liver , 293T

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MPST. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950946

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mercaptopyruvate sulfurtransferase , MST , TST2 , TUM1

Cellular Localization

Cytoplasm

Background

This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MPST antibody [C2C3], C-term used in Western Blot (WB). GTX108274

GTX108274 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MPST antibody [C2C3], C-term (GTX108274) diluted at 1:500.

Anti-MPST antibody [C2C3], C-term used in Western Blot (WB). GTX108274

GTX108274 WB Image

KO/KD

Wild-type (WT) and MPST knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MPST antibody [C2C3], C-term (GTX108274) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MPST antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108274

GTX108274 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using MPST(GTX108274) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MPST antibody [C2C3], C-term used in Western Blot (WB). GTX108274

GTX108274 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MPST antibody [C2C3], C-term (GTX108274) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-MPST antibody [C2C3], C-term used in Western Blot (WB). GTX108274

GTX108274 WB Image

The data was published in the 2022 in Biomolecules. PMID: 35204649

REFERENCE

Application Reference

Rydz L et al. Biomolecules 2023; 13 (10) Hypoxia-Induced Changes in L-Cysteine Metabolism and Antioxidative Processes in Melanoma Cells.
Zhang J et al. Redox Biol 2022; 56 : 102469 MPST deficiency promotes intestinal epithelial cell apoptosis and aggravates inflammatory bowel disease via AKT.
Application : WB, IHC-P
Reactivity : Human, Mouse
Halina Jurkowska et al. Biomolecules 2022; 12 (2) Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines.
Application : WB
Reactivity : Human
Bronowicka-Adamska P et al. Int J Mol Sci 2020; 21 (3) Effect of S-Allyl -L-Cysteine on MCF-7 Cell Line 3-Mercaptopyruvate Sulfurtransferase/Sulfane Sulfur System, Viability and Apoptosis.
Reactivity : Human
Wang YH et al. EMBO Rep 2019; : e45986 Dysregulation of cystathionine 帠-lyase promotes prostate cancer progression and metastasis.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169