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MRI1 antibody [6A10]

Overexpression
Anti-MRI1 antibody [6A10] used in IHC (Paraffin sections) (IHC-P). GTX84086
Anti-MRI1 antibody [6A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84086
Anti-MRI1 antibody [6A10] used in Western Blot (WB). GTX84086
Anti-MRI1 antibody [6A10] used in IHC (Paraffin sections) (IHC-P). GTX84086
Anti-MRI1 antibody [6A10] used in Western Blot (WB). GTX84086
Anti-MRI1 antibody [6A10] used in IHC (Paraffin sections) (IHC-P). GTX84086
Anti-MRI1 antibody [6A10] used in IHC (Paraffin sections) (IHC-P). GTX84086
Anti-MRI1 antibody [6A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84086

Cat. No. GTX84086

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6A10

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all MRI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:50-1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human MRI1 (NP_115661) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methylthioribose-1-phosphate isomerase 1 , M1Pi , MRDI , MTNA , Ypr118w

Cellular Localization

Nucleus,Cytoplasm

Background

Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P). Independently from catalytic activity, promotes cell invasion in response to constitutive RhoA activation by promoting FAK tyrosine phosphorylation and stress fiber turnover.

Database

Research Area

DATA IMAGES

Anti-MRI1 antibody [6A10] used in IHC (Paraffin sections) (IHC-P). GTX84086

GTX84086 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84086 MRI1 antibody [6A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MRI1 antibody [6A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84086

GTX84086 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with MRI1 plasmid using GTX84086 MRI1 antibody [6A10].

Anti-MRI1 antibody [6A10] used in Western Blot (WB). GTX84086

GTX84086 WB Image

Overexpression

WB analysis of HEK293T cells transfected with MRI1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84086 MRI1 antibody [6A10].
Loading : 5 ug per lane

Anti-MRI1 antibody [6A10] used in IHC (Paraffin sections) (IHC-P). GTX84086

GTX84086 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84086 MRI1 antibody [6A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MRI1 antibody [6A10] used in Western Blot (WB). GTX84086

GTX84086 WB Image

WB analysis of various cell lines using GTX84086 MRI1 antibody [6A10].
Loading : 35 ug per lane
Dilution : 1:200

Anti-MRI1 antibody [6A10] used in IHC (Paraffin sections) (IHC-P). GTX84086

GTX84086 IHC-P Image

IHC-P analysis of human breast tissue using GTX84086 MRI1 antibody [6A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MRI1 antibody [6A10] used in IHC (Paraffin sections) (IHC-P). GTX84086

GTX84086 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84086 MRI1 antibody [6A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MRI1 antibody [6A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84086

GTX84086 ICC/IF Image

ICC/IF analysis of A549 cells using GTX84086 MRI1 antibody [6A10].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519