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MRLC2 antibody

Comparison
Anti-MRLC2 antibody used in Western Blot (WB). GTX102091
Anti-MRLC2 antibody used in Western Blot (WB). GTX102091

Cat. No. GTX102091

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MRLC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

K562 , Jurkat , Raji , NCI-H929

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRLC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241070

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin light chain 12B , MLC-B , MRLC2

Background

The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-MRLC2 antibody used in Western Blot (WB). GTX102091

GTX102091 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with MRLC2 antibody (GTX102091) diluted at 1:5000 and competitor's antibody (SC-15370) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-MRLC2 antibody used in Western Blot (WB). GTX102091

GTX102091 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with MRLC2 antibody (GTX102091) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169