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MRPS16 antibody

Anti-MRPS16 antibody used in Western Blot (WB). GTX87139
Anti-MRPS16 antibody used in IHC (Paraffin sections) (IHC-P). GTX87139

Cat. No. GTX87139

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPS16 (81-130).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CGI132 , COXPD2 , MRPS16 , RPMS16 , mitochondrial ribosomal protein S16

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S16P family. The encoded protein is one of the most highly conserved ribosomal proteins between mammalian and yeast mitochondria. Three pseudogenes (located at 8q21.3, 20q13.32, 22q12-q13.1) for this gene have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MRPS16 antibody used in Western Blot (WB). GTX87139

GTX87139 WB Image

WB analysis of HepG2 cell lysates using GTX87139 MRPS16 antibody. The lane on the right is blocked with the synthesized peptide.

Anti-MRPS16 antibody used in IHC (Paraffin sections) (IHC-P). GTX87139

GTX87139 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX87139 MRPS16 antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399