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MST1 (phospho Thr183) + MST2 (phospho Thr180) antibody

Orthogonal
Anti-MST1 (phospho Thr183) + MST2 (phospho Thr180) antibody used in Western Blot (WB). GTX133948

Cat. No. GTX133948

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MST1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HeLa (1 μM Staurosporine treatment for 3 hr)

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr183 of human MST1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887150

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine/threonine kinase 4 , KRS2 , MST1 , YSK3

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MST1 (phospho Thr183) + MST2 (phospho Thr180) antibody used in Western Blot (WB). GTX133948

GTX133948 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MST1 (phospho Thr183) + MST2 (phospho Thr180) antibody (GTX133948) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Byung-Cheon Jeong et al. Nat Struct Mol Biol 2021; 28 (3) : 290-299 Cryo-EM structure of the Hippo signaling integrator human STRIPAK.
Application : WB
Reactivity : Human
Bae SJ et al. Elife 2020; 9 STK25 suppresses Hippo signaling by regulating SAV1-STRIPAK antagonism.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169