MTH1 antibody [AT3B3]

Cat. No. GTX57631

Host	Mouse
Clonality	Monoclonal
Clone Name	AT3B3
Isotype	IgG1
Application	WB, ICC/IF, FACS
Reactivity	Human
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX57631 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT3B3 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NUDT3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nudix hydrolase 1 , MTH1 , NUDT1

Cellular Localization

Cytoplasm,Mitochondrion matrix,Nucleus

Background

Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A rare single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq, Dec 2018]

Database

Gene ID: 4521 NUDT1
UniProt: P36639 NUDT1

Research Area

DATA IMAGES

GTX57631 FACS Image

FACS analysis of Jurkat cells using GTX57631 MTH1 antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

GTX57631 ICC/IF Image

ICC/IF analysis of A549 cells using GTX57631 MTH1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

GTX57631 WB Image

WB analysis of various samples using GTX57631 MTH1 antibody.
Lane 1 : K562 whole cell lysate
Lane 2 : SW480 whole cell lysate
Lane 3 : THP-1 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : A549 whole cell lysate
Loading : 40 μg
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty