MTHFD2 antibody [N1C3]

Name: MTHFD2 antibody [N1C3]
Brand: GeneTex
SKU: GTX104990
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-MTHFD2 antibody [N1C3] used in Western Blot (WB). GTX104990

Anti-MTHFD2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104990

Anti-MTHFD2 antibody [N1C3] used in Immunoprecipitation (IP). GTX104990

Anti-MTHFD2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104990

Cat. No. GTX104990

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all MTHFD2 products

View product citations for antibody GTX104990 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MTHFD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950954

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase , NMDMC

Cellular Localization

Mitochondrion

Background

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq]

Database

Gene ID: 10797 MTHFD2
UniProt: P13995 MTHFD2

Research Area

DATA IMAGES

GTX104990 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104990 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104990 IHC-P Image

MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human MCF7 xenograft.
MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104990 IP Image

Immunoprecipitation of MTHFD2 protein from 293T whole cell extracts using 5 μg of MTHFD2 antibody [N1C3] (GTX104990).
Western blot analysis was performed using MTHFD2 antibody [N1C3] (GTX104990).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX104990 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using MTHFD2(GTX104990) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104990 ICC/IF Image

MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MTHFD2 stained by MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX104990 IHC-P Image

MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104990 IHC-P Image

MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
MTHFD2 stained by MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Gao Y et al. J Biol Chem 2023; 299 (7) : 104909 Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.

Lee WD et al. Cell Metab 2021; 33 (1) : 190-198.e6 Tumor Reliance on Cytosolic versus Mitochondrial One-Carbon Flux Depends on Folate Availability.

Zhang J et al. BMC Cancer 2019; 19 (1) : 921 Lentivirus-mediated CDglyTK gene-modified free flaps by intra-artery perfusion show targeted therapeutic efficacy in rat model of breast cancer.

Application : WB

Reactivity : Human

Costas Koufaris et al. Cancer & Metabolism 2018; 6 : 12 Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.

Application : IP

Reactivity : Human

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