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MUSK antibody

Anti-MUSK antibody used in IHC (Paraffin sections) (IHC-P). GTX25619
Anti-MUSK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25619
Anti-MUSK antibody used in IHC (Paraffin sections) (IHC-P). GTX25619
Anti-MUSK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25619
Anti-MUSK antibody used in Western Blot (WB). GTX25619
Anti-MUSK antibody used in Western Blot (WB). GTX25619
Anti-MUSK antibody used in IHC (Paraffin sections) (IHC-P). GTX25619

Cat. No. GTX25619

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, IHC

Reactivity

 Human, Mouse, Rat
Package
200 μl ($399)
See all MUSK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:20-1:100
IHC-P 1:20-1:100
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant protein containing residues 210-304 from the extracellular domain of rat MuSK

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

muscle, skeletal, receptor tyrosine kinase , Mdk4 , Mlk , Nsk1 , Nsk2 , Nsk3

Background

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MUSK antibody used in IHC (Paraffin sections) (IHC-P). GTX25619

GTX25619 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX25619 MUSK antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes using a microwave
Permeabilization : TBS/0.025% Triton
Dilution : 1:20

Anti-MUSK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25619

GTX25619 ICC/IF Image

ICC/IF analysis of L6 cells using GTX25619 MUSK antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:20 in blocking buffer for at least 1 hour at room temperature

Anti-MUSK antibody used in IHC (Paraffin sections) (IHC-P). GTX25619

GTX25619 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX25619 MUSK antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes using a microwave
Permeabilization : TBS/0.025% Triton
Dilution : 1:20

Anti-MUSK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25619

GTX25619 ICC/IF Image

ICC/IF analysis of C2C12 cells using GTX25619 MUSK antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:20 in blocking buffer for at least 1 hour at room temperature

Anti-MUSK antibody used in Western Blot (WB). GTX25619

GTX25619 WB Image

WB analysis of 30 ug of rat skeletal muscle tissue lysate using GTX25619 MUSK antibody.
Dilution : 1 μg/ml

Anti-MUSK antibody used in Western Blot (WB). GTX25619

GTX25619 WB Image

WB analysis of 3ng of muscle-specific kinase antigen using GTX25619 MUSK antibody.
Dilution : 1 μg/ml

Anti-MUSK antibody used in IHC (Paraffin sections) (IHC-P). GTX25619

GTX25619 IHC-P Image

IHC-P analysis of rat skeletal muscle tissue using GTX25619 MUSK antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes using a microwave
Permeabilization : TBS/0.025% Triton
Dilution : 1:20

REFERENCE

Application Reference

Wu S et al. J Neuropathol Exp Neurol 2022; 81 (10) : 816-824 Inhibiting Matrix Metalloproteinases Protects Evoked Electromyography Amplitudes and Muscle Tension in the Orbicularis Oris Muscle in a Rat Model of Facial Nerve Injury.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399