APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Rat2 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine, Dog, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.43 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human MYH9. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2037478
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
myosin heavy chain 9 , BDPLT6 , DFNA17 , EPSTS , FTNS , MATINS , MHA , NMHC-II-A , NMMHC-IIA , NMMHCA
Background
This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113236 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113236 IHC-P Image
MYH9 antibody [N1-2], N-term detects MYH9 protein at cytoplasm in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113236 ICC/IF Image
MYH9 antibody [N1-2], N-term detects MYH9 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: MYH9 stained by MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX113236 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113236 IP Image
Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 μg of MYH9 antibody [N1-2], N-term (GTX113236). Western blot analysis was performed using MYH9 antibody [N1-2], N-term (GTX113236). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX113236 WB Image
MYH9 antibody [N1-2], N-term detects MYH9 protein by western blot analysis. A. 30 μg Rat2 whole cell lysate/extract 5% SDS-PAGE MYH9 antibody [N1-2], N-term (GTX113236) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113236 IHC-P Image
MYH9 antibody [N1-2], N-term detects MYH9 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113236 WB Image
MYH9 antibody [N1-2], N-term detects MYH9 protein by western blot analysis. A. 30 μg NIH-3T3 whole cell lysate/extract B. 30 μg BCL-1 whole cell lysate/extract C. 30 μg Raw264.7 whole cell lysate/extract D. 30 μg C2C12 whole cell lysate/extract 5% SDS-PAGE MYH9 antibody [N1-2], N-term (GTX113236) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
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