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MYPT1 antibody

Anti-MYPT1 antibody used in Flow cytometry (FACS). GTX12027
Anti-MYPT1 antibody used in Western Blot (WB). GTX12027
Anti-MYPT1 antibody used in Flow cytometry (FACS). GTX12027

Cat. No. GTX12027

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($439)
See all MYPT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
FACS 1-3μg/1x10⁶cells
Not tested in other applications.

Calculated MW

115.28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PPP1R12A (1-17aa MKMADAKQKRNEQLKRW), identical to the related rat and mouse sequences.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cytoplasm

Background

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-MYPT1 antibody used in Flow cytometry (FACS). GTX12027

GTX12027 FACS Image

FACS analysis of U251 cells using GTX12027 MYPT1 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Cell only control
Antibody amount : 1μg/1x10⁶ cells for 30 min at 20ºC

Anti-MYPT1 antibody used in Western Blot (WB). GTX12027

GTX12027 WB Image

WB analysis of various samples using GTX12027 MYPT1 antibody.
Lane 1 : rat liver tissue lysate at 50ug
Lane 2 : 293T whole cell lysate at 40ug
Lane 3 : HeLa whole cell lysate at 40ug
Dilution : 0.5 μg/mL

Anti-MYPT1 antibody used in Flow cytometry (FACS). GTX12027

GTX12027 FACS Image

FACS analysis of HeLa cells using GTX12027 MYPT1 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Cell only control
Antibody amount : 1μg/1x10⁶ cells for 30 min at 20ºC

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 439