APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:300 |
|
Note :
IHC-P
The recommended fixative is 10% neutral-buffered formalin for paraffin-embedded tissue sections. Heat Induced Epitope Retreival (HIER) was performed in Epitope Retreival Solution pH6.
|
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
Tissue culture supernatant
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Antigen Species
Human
Immunogen
Prokaryotic recombinant protein corresponding to the unbound region of the human mast cell tryptase molecule.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tryptase alpha/beta 1 , TPS1 , TPS2 , TPSB1 , TPSB2 , Tryptase-2
Cellular Localization
Secreted
Background
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Mast cell tryptase antibody [10D11] used in IHC (Paraffin sections) (IHC-P). GTX01964](/templates/default/js/lightbox/images/loading.gif "Anti-Mast cell tryptase antibody [10D11] used in IHC (Paraffin sections) (IHC-P). GTX01964")
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GTX01964 IHC-P Image
IHC-P analysis of human skin using GTX01964 Mast cell tryptase antibody [10D11]. Note cytoplasmic staining of mast cells.
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REFERENCE
There are currently no references for Mast cell tryptase antibody [10D11] (GTX01964). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Mast cell tryptase antibody [10D11] (GTX01964). Be the first to share your experience with this product.
![Anti-Mast cell tryptase antibody [10D11] used in IHC (Paraffin sections) (IHC-P). GTX01964](/upload/website/prouct_img/small/GTX01964/GTX01964_20200811_IHC-P_109_w_23053121_992.webp "Anti-Mast cell tryptase antibody [10D11] used in IHC (Paraffin sections) (IHC-P). GTX01964")
![Anti-Mast cell tryptase antibody [10D11] used in IHC (Paraffin sections) (IHC-P). GTX01964](/upload/website/prouct_img/normal/GTX01964/GTX01964_20200811_IHC-P_109_w_23053121_992.webp "Anti-Mast cell tryptase antibody [10D11] used in IHC (Paraffin sections) (IHC-P). GTX01964")