APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended.
|
Not tested in other applications.
Positive Control
Mast cells containing tissue or tumors
PROPERTIES
Form
Liquid
Buffer
Tris-HCl buffer, 0.2% BSA
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human Tryptase protein fragment (around aa 115-233)
Purification
Purified IgG
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tryptase alpha/beta 1 , TPS1 , TPS2 , TPSB1 , TPSB2 , Tryptase-2
Cellular Localization
Secreted
Background
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]
Database
Research Area
REFERENCE
There are currently no references for Mast cell tryptase antibody [ZM96] (GTX01844). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Mast cell tryptase antibody [ZM96] (GTX01844). Be the first to share your experience with this product.
