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Melanoma gp100 antibody [7B2]

Anti-Melanoma gp100 antibody [7B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83639
Anti-Melanoma gp100 antibody [7B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83639
Anti-Melanoma gp100 antibody [7B2] used in Flow cytometry (FACS). GTX83639

Cat. No. GTX83639

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7B2

Isotype

 IgG1

Application

 ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($519)
See all Melanoma gp100 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SILV (NP_008859) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

premelanosome protein , D12S53E , ME20 , ME20-M , ME20M , P1 , P100 , PMEL17 , SI , SIL , SILV , gp100

Cellular Localization

Golgi apparatus

Background

Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.

Database

Research Area

DATA IMAGES

Anti-Melanoma gp100 antibody [7B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83639

GTX83639 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Melanoma gp100 plasmid using GTX83639 Melanoma gp100 antibody [7B2].

Anti-Melanoma gp100 antibody [7B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83639

GTX83639 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX83639 Melanoma gp100 antibody [7B2].

Anti-Melanoma gp100 antibody [7B2] used in Flow cytometry (FACS). GTX83639

GTX83639 FACS Image

FACS analysis of HEK293T cells transfected with either Melanoma gp100 plasmid(Red) or empty vector control plasmid(Blue) using GTX83639 Melanoma gp100 antibody [7B2].
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519