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Mer2 (phospho Ser30) antibody

Anti-Mer2 (phospho Ser30) antibody used in Western Blot (WB). GTX48730
Anti-Mer2 (phospho Ser30) antibody used in Western Blot (WB). GTX48730

Cat. No. GTX48730

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA

Reactivity

 Saccharomyces cerevisiae
Package
50 μg ($399)
See all Mer2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IP 1:100
ELISA 1:5000-1:25000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Saccharomyces cerevisiae

Immunogen

Synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rec107p , MER2

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-Mer2 (phospho Ser30) antibody used in Western Blot (WB). GTX48730

GTX48730 WB Image

Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phos-phorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphorylated protein. The primary antibody was used at a 1:5,000 dilution.

Anti-Mer2 (phospho Ser30) antibody used in Western Blot (WB). GTX48730

GTX48730 WB Image

Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 in whole cell extracts.  Cells were either wild type (+) or contained mer2 deletions (D). Extracts were prepared from cells 4hr after initiation of meiosis.  Proteins were obtained using TCA precipitation.  The primary antibody was used at a 1:7,500 dilution.  Secondary antibody was used at 1:5,000 dilution.  Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399