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Microcephalin antibody

Anti-Microcephalin antibody used in Western Blot (WB). GTX03494
Anti-Microcephalin antibody used in IHC (Paraffin sections) (IHC-P). GTX03494
Anti-Microcephalin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03494
Anti-Microcephalin antibody used in IHC (Paraffin sections) (IHC-P). GTX03494

Cat. No. GTX03494

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($409)
See all Microcephalin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Predict Reactivity

Rabbit, Dog, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human MCPH1(Accession Q8NEM0), corresponding to amino acid residues C110-M134.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microcephalin 1 , BRIT1 , MCT

Cellular Localization

Centrosome

Background

This gene encodes a DNA damage response protein. The encoded protein may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in this gene have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-Microcephalin antibody used in Western Blot (WB). GTX03494

GTX03494 WB Image

WB analysis of K562 cell lysate using GTX03494 Microcephalin antibody. The lane on the left was treated with the antigen-specific peptide.

Anti-Microcephalin antibody used in IHC (Paraffin sections) (IHC-P). GTX03494

GTX03494 IHC-P Image

IHC-P analysis of rat brain tissue using GTX03494 Microcephalin antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Microcephalin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03494

GTX03494 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX03494 Microcephalin antibody.
Red : Primary antibody
Green : beta-tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-Microcephalin antibody used in IHC (Paraffin sections) (IHC-P). GTX03494

GTX03494 IHC-P Image

IHC-P analysis of rat testis tissue using GTX03494 Microcephalin antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 409