APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 20 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Raji Cell Slide(GTX25538) , Raji Cell Lysate(GTX27908)
Product Note
This antibody is specific for Mimitin Internal
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Mimitin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Mimitin.The immunogen is located within the last 50 amino acids of Mimitin.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NADH:ubiquinone oxidoreductase complex assembly factor 2 , B17.2L , MC1DN10 , MMTN , NDUFA12L , mimitin
Cellular Localization
Mitochondrion
Background
Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-kB-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.
Database
Research Area
DATA IMAGES
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GTX85237 ICC/IF Image
ICC/IF analysis of Raji cells using GTX85237 Mimitin antibody.
Working concentration : 20 μg/ml
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GTX85237 WB Image
WB analysis of Raji cell lysate using GTX85237 Mimitin antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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REFERENCE
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REVIEW
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