APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-1.5μg/ml |
| 5μg/ml |
|
Note :
IHC-P
Human Liver, expression of MGLL is detected in hepatocytes.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88653-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_009214.1; NP_001003794.1).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QDLPHLVNADGQY, from the internal region (near N Terminus) of the protein sequence according to NP_009214.1; NP_001003794.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
monoglyceride lipase , HU-K5 , HUK5 , MAGL , MGL
Cellular Localization
Cytoplasm, cytosol , Membrane
Background
This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
Database
Research Area
DATA IMAGES
|
GTX88653 IHC-P Image
IHC-P analysis of human liver using GTX88653 Monoglyceride lipase antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
|
|
|
GTX88653 WB Image
WB analysis of human frontal cortex (A) and mouse adipose (B) lysates using GTX88653 Monoglyceride lipase antibody, Internal (near N-term).
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for Monoglyceride lipase antibody, Internal (near N-term) (GTX88653). Be the first to share your publications with this product.
REVIEW
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