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Mouse GM-CSF protein, His and GST tag (active)

Mouse GM-CSF protein, His and GST tag (active). GTX00282-pro
Mouse GM-CSF protein, His and GST tag (active). GTX00282-pro
Mouse GM-CSF protein, His and GST tag (active). GTX00282-pro

Cat. No. GTX00282-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($259)
See all GM-CSF protein products

APPLICATION

Application Note

Measured in a cell proliferation assay using mouse BMDC (bone marrow derived dendritic cells). The ED₅₀ (median effective dose) for this effect is less than 0. 25 ng/ml. This protein used in cellular experiment in Six-eight weeks old Balb/c mice. At first, mouse femur and tibia were taken out, and then bone marrow was washed out with serum-free RPMI-1640 medium, followed by centrifugation at 1200RPM for 5min (4ºC). ACK buffer was added to get rid of red blood cells, and then, centrifuged at 1200RPM for 5min (4ºC). Cell pellets were collected and re-suspended, the cells were cultured in DMEM medium supplemented with 10% FBS, or DMEM medium supplemented with 10% FBS and GMCSF at 37ºC with 5% CO₂ in thermostatic incubator. Three days later, cells were observed by microscope.

Observed MW

44 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Pro19~Lys141 (NP_034099.2)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

colony stimulating factor 2 (granulocyte-macrophage) , CSF , Csfgm , GMCSF , Gm-CSf , MGI-IGM

Database

Research Area

DATA IMAGES

Mouse GM-CSF protein, His and GST tag (active). GTX00282-pro

GTX00282-pro Functional Assay Image

Cell proliferation effect of GTX00282-pro Mouse GM-CSF protein (active).
(A) BMDC cultured in DMEM supplemented with 10% FBS.
(B) BMDC cultured in DMEM supplemented with 10% FBS and GMCSF.

Mouse GM-CSF protein, His and GST tag (active). GTX00282-pro

GTX00282-pro Image

SDS-PAGE analysis of GTX00282-pro Mouse GM-CSF protein (active).

Mouse GM-CSF protein, His and GST tag (active). GTX00282-pro

GTX00282-pro Image

WB analysis of GTX00282-pro Mouse GM-CSF protein (active).

REFERENCE

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SDS
Proclin.pdf
Package List Price ($)
$ 259