GeneTex
United States (US)

Mouse IgG2c antibody (HRP)

Mouse-IgG2c-antibody-HRP-GTX77297-IHC-1_18121411_745.jpg

Cat No. GTX77297

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA, IHC
Reactivity Mouse
APPLICATION

Application Note

Recommended Starting Dilutions:
WB: 1:1000-1:10,000;
ELISA: 1:10,000-1:30,000. Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

This product reacts with IgG 2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice. The anti IgG 2c does not react with L-chains or IgG 2a, IgG 2b, IgG 1 or IgG3.This reagent may also be used where a reagent specific for Igh 1-b allele is indicated. (Reference: J. Immunological Methods 212: 187-192, 1998).
PROPERTIES

Form

Liquid

Buffer

50 mM HEPES pH7.0, 0.1M Sodium Chloride with 0.2% BSA, 0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Goats were immunized with highly purified IgG 2c isolated from C57BL/6 mice, in Freund's adjuvant.

Target Isotype

IgG2c

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mouse IgG2c secondary antibody, Goat antiMouse IgG2c HRP, Goat anti-Mouse IgG2c HRP, anti-Mouse IgG2c antibody, antiMouse IgG2c antibody, Goat anti Mouse IgG2c HRP, anti Mouse IgG2c antibody

Background

Immunoglobulin G (IgG), is one of the most abundant protein in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
DATA IMAGES
Mouse-IgG2c-antibody-HRP-GTX77297-IHC-1_18121411_745.jpg

GTX77297 IHC Image


REFERENCE
Package List Price ($)
$ 179