Recommended Starting Dilutions:
ELISA: 1:10,000-1:30,000. Optimal dilutions/concentrations should be determined by the end user.
This product reacts with IgG 2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice. The anti IgG 2c does not react with L-chains or IgG 2a, IgG 2b, IgG 1 or IgG3.This reagent may also be used where a reagent specific for Igh 1-b allele is indicated. (Reference: J. Immunological Methods 212: 187-192, 1998).
50 mM HEPES pH7.0, 0.1M Sodium Chloride with 0.2% BSA, 0.01% Thimerosal
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Goats were immunized with highly purified IgG 2c isolated from C57BL/6 mice, in Freund's adjuvant.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Mouse IgG2c secondary antibody, Goat antiMouse IgG2c HRP, Goat anti-Mouse IgG2c HRP, anti-Mouse IgG2c antibody, antiMouse IgG2c antibody, Goat anti Mouse IgG2c HRP, anti Mouse IgG2c antibody
Immunoglobulin G (IgG), is one of the most abundant protein in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.