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Mouse lamda Light Chain antibody [RM110]

Mouse lamda Light Chain antibody [RM110]. GTX60857
Mouse lamda Light Chain antibody [RM110]. GTX60857
Mouse lamda Light Chain antibody [RM110]. GTX60857

Cat No. GTX60857

Host Rabbit
Clonality Monoclonal
Clone Name RM110
Isotype IgG
Application WB, ICC/IF, FACS, IP, ELISA, IHC
Reactivity Mouse
Package
100 μg ($279)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody reacts to the lamda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG.
PROPERTIES

Form

Liquid

Buffer

PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgM lambda

Target Isotype

IgG (lambda light chain)

Purification

Protein A affinity purified from an animal origin–free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Igl-C1 Antibody , immunoglobulin lambda chain, constant region 1 Antibody , IgL Antibody , Clambda1 Antibody
DATA IMAGES
Mouse lamda Light Chain antibody [RM110]. GTX60857

GTX60857 ELISA Image

A titer ELISA of mouse IgG1λ. The plate was coated with different amounts of mouse IgG1λ. A serial dilution of RM110 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Mouse lamda Light Chain antibody [RM110]. GTX60857

GTX60857 ELISA Image

ELISA of mouse immunoglobulins shows RM110 reacted to the lamda light chain of mouse immunoglobulins. No cross reactivity with the kappalight chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM110 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Mouse lamda Light Chain antibody [RM110]. GTX60857

GTX60857 WB Image

Western blot of nonreduced(-) and reduced(+) mouse IgG1κ and IgG1λ (20ng/lane), using 0.2ug/mL of clone RM110. This antibody reacted to nonreduced IgG1λ.

REFERENCE
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REVIEW
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Package List Price ($)
$ 279