GeneTex

  • Country / Location Selection

United States (US)

Mucin 5AC antibody [45M1]

Cat. No. GTX11335

Reference 9

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 45M1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Rabbit, Cat, Chicken, Pig, Monkey, Hedgehog
Package
100 μl ($339)
See all Mucin 5AC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:200
Not tested in other applications.

Calculated MW

586 kDa. ( Note )

Positive Control

human stomach

Product Note

The antibody recognizes the mucin epitope g located in the peptide core of gastric mucin (>1,000 kDa). This epitope is completely destroyed by thiol reduction (using mercaptoethanol) and partially lost following trypsin proteolysis, but is stable upon periodate oxidation. The antibody stains the surface gastric epithelium of normal human gastrointestinal tract and reacts with fetal, pre-cancerous and cancerous colonic mucosa, but not with normal colon.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

mucin from human ovarian cyst fluid.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_372411

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mucin 5AC, oligomeric mucus/gel-forming , MUC5 , TBM , leB , mucin

Cellular Localization

Secreted

Background

Mucin is a high M.W. (>1,000 kDa) glycoprotein, expressed by mucus cells of the gastric epithelium and by goblet cells of the fetal, precancerous and cancerous colon, but not by those of the normal colon. It also appears in other epithelial tissues, which are embryologically derived from the foregut (epigastric and bronchial epithelium) and in Muller ducts (mucus cells of the endocervix and urethral epithelium near the prostatic utriculus).

Database

Research Area

REFERENCE

Application Reference

Hu CM et al. Adv Sci (Weinh) 2023; 10 (36) : e2301240 Oncogenic KRAS, Mucin 4, and Activin A-Mediated Fibroblast Activation Cooperate for PanIN Initiation.
Xue Y et al. J Asthma Allergy 2021; 14 : 883-896 Small-Airway Dysfunction is Involved in the Pathogenesis of Asthma: Evidence from Two Mouse Models.
Application : IHC-P
Reactivity : Mouse
Yang DQ et al. Oxid Med Cell Longev 2021; 2021 : 6644238 Mitochondrial-Targeting Antioxidant SS-31 Suppresses Airway Inflammation and Oxidative Stress Induced by Cigarette Smoke.
Application : IHC-P
Yang D et al. Int Immunopharmacol 2021; 90 : 107149 Mitoquinone ameliorates cigarette smoke-induced airway inflammation and mucus hypersecretion in mice.
Application : IHC-P
Reactivity : Mouse

Clone Reference

Tamura M et al. Biol Pharm Bull 2020; 43 (2):356-360 Potential Interaction between Galectin-2 and MUC5AC in Mouse Gastric Mucus.
Show More +
Submit a Reference

REVIEW

There are currently no reviews for Mucin 5AC antibody [45M1] (GTX11335). Be the first to share your experience with this product.
Submit a Review
Application Reference
Hu CM et al. Adv Sci (Weinh) 2023; 10 (36):e2301240 Oncogenic KRAS, Mucin 4, and Activin A-Mediated Fibroblast Activation Cooperate for PanIN Initiation.
Xue Y et al. J Asthma Allergy 2021; 14:883-896 Small-Airway Dysfunction is Involved in the Pathogenesis of Asthma: Evidence from Two Mouse Models.
Application : IHC-P
Reactivity : Mouse
Yang DQ et al. Oxid Med Cell Longev 2021; 2021:6644238 Mitochondrial-Targeting Antioxidant SS-31 Suppresses Airway Inflammation and Oxidative Stress Induced by Cigarette Smoke.
Application : IHC-P
Yang D et al. Int Immunopharmacol 2021; 90:107149 Mitoquinone ameliorates cigarette smoke-induced airway inflammation and mucus hypersecretion in mice.
Application : IHC-P
Reactivity : Mouse
Yang Z et al. Front Pharmacol 2020; 11:607384 Tsantan Sumtang Restored Right Ventricular Function in Chronic Hypoxia-Induced Pulmonary Hypertension Rats.
Application : IHC-P
Reactivity : Rat
Jing L et al. J Ethnopharmacol 2019; 235:424-434 Srolo Bzhtang, a traditional Tibetan medicine formula, inhibits cigarette smoke induced airway inflammation and muc5ac hypersecretion via suppressing IL-13/STAT6 signaling pathway in rats.
Application : WB, IHC-P
Reactivity : Rat
Clone Reference
Tamura M et al. Biol Pharm Bull 2020; 43 (2):356-360 Potential Interaction between Galectin-2 and MUC5AC in Mouse Gastric Mucus.
Nagaoka K et al. Environ Toxicol 2019; (Epub) Human albumin augmented airway inflammation induced by PM2.5 in NC/Nga mice.
Wei Peng et al. Pharmacognosy Magazine 2019; 15 (60):29-35 Comparative research of the curative effects of pinelliae rhizoma and pinelliae rhizoma praeparatum cum alumine on ovalbumin-induced allergic asthma in rats.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 339