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Myelin basic protein antibody [2H9]

Anti-Myelin basic protein antibody [2H9] used in IHC (Paraffin sections) (IHC-P). GTX60519
Anti-Myelin basic protein antibody [2H9] used in Western Blot (WB). GTX60519
Anti-Myelin basic protein antibody [2H9] used in IHC (Paraffin sections) (IHC-P). GTX60519
Anti-Myelin basic protein antibody [2H9] used in Western Blot (WB). GTX60519
Anti-Myelin basic protein antibody [2H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60519
Anti-Myelin basic protein antibody [2H9] used in ELISA (ELISA). GTX60519
Anti-Myelin basic protein antibody [2H9] used in Flow cytometry (FACS). GTX60519

Cat. No. GTX60519

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2H9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($419)
See all Myelin basic protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human MBP expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myelin basic protein

Background

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Myelin basic protein antibody [2H9] used in IHC (Paraffin sections) (IHC-P). GTX60519

GTX60519 IHC-P Image

IHC-P analysis of human brain tissue using GTX60519 Myelin basic protein antibody [2H9].

Anti-Myelin basic protein antibody [2H9] used in Western Blot (WB). GTX60519

GTX60519 WB Image

WB analysis of HEK293 (1) and MBP-hIgGFc transfected HEK293 (2) cell lysate using GTX60519 Myelin basic protein antibody [2H9].

Anti-Myelin basic protein antibody [2H9] used in IHC (Paraffin sections) (IHC-P). GTX60519

GTX60519 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX60519 Myelin basic protein antibody [2H9].

Anti-Myelin basic protein antibody [2H9] used in Western Blot (WB). GTX60519

GTX60519 WB Image

WB analysis of human MBP (AA: 1-197) recombinant protein using GTX60519 Myelin basic protein antibody [2H9].

Anti-Myelin basic protein antibody [2H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60519

GTX60519 ICC/IF Image

ICC/IF analysis of MSCS cells using GTX60519 Myelin basic protein antibody [2H9].
Green : Myelin basic protein
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Myelin basic protein antibody [2H9] used in ELISA (ELISA). GTX60519

GTX60519 ELISA Image

ELISA analysis of antigen using GTX60519 Myelin basic protein antibody [2H9].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Myelin basic protein antibody [2H9] used in Flow cytometry (FACS). GTX60519

GTX60519 FACS Image

FACS analysis of HepG2 cells using GTX60519 Myelin basic protein antibody [2H9].
Green : Myelin basic protein
Red : negative control

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419