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Myl7 antibody

Anti-Myl7 antibody used in IHC (Whole mount) (IHC-Wm). GTX128346
Anti-Myl7 antibody used in Western Blot (WB). GTX128346
Anti-Myl7 antibody used in IHC (Whole mount) (IHC-Wm). GTX128346
Anti-Myl7 antibody used in IHC (Whole mount) (IHC-Wm). GTX128346

Cat. No. GTX128346

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IHC-Wm

Reactivity

 Zebrafish, Japanese Medaka
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Myl7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:50000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Positive Control

zebrafish adult muscle

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish myl7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885759

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin, light chain 7, regulatory , cmlc2 , mylc2a , zgc:92755

Database

Research Area

DATA IMAGES

Anti-Myl7 antibody used in IHC (Whole mount) (IHC-Wm). GTX128346

GTX128346 IHC-Wm Image

myl7 antibody detects myl7 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
myl7 antibody (GTX128346) dilution: 1:100.
Anti-Myl7 antibody used in Western Blot (WB). GTX128346

GTX128346 WB Image

myl7 antibody detects myl7 protein by Western blot analysis.
A. 10 μg zebrafish adult muscle lysate/extract
15 % SDS-PAGE
myl7 antibody (GTX128346) dilution: 1:50000

Anti-Myl7 antibody used in IHC (Whole mount) (IHC-Wm). GTX128346

GTX128346 IHC-Wm Image

Myl7 antibody detects Myl7 antibody protein at cardiac and skeletal muscles on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Myl7 antibody (GTX128346) dilution: 1:200.
Anti-Myl7 antibody used in IHC (Whole mount) (IHC-Wm). GTX128346

GTX128346 IHC-Wm Image

Myl7 antibody detects Myl7 protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Myl7 antibody (GTX128346) dilution: 1:100.

REFERENCE

Application Reference

Oudhoff H et al. NPJ Regen Med 2024; 9 (1) : 8 Skeletal muscle regeneration after extensive cryoinjury of caudal myomeres in adult zebrafish.
Chen W et al. Hum Genomics 2022; 16 (1) : 48 Biallelic mutations of TTC12 and TTC21B were identified in Chinese patients with multisystem ciliopathy syndromes.
Bertozzi A et al. Dev Biol 2020; (Epub) Is zebrafish heart regeneration "complete"? Lineage-restricted cardiomyocytes proliferate to pre-injury numbers but some fail to differentiate in fibrotic hearts.
Application : IHC-Fr
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169