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Myosin Light Chain 2 (MLC-2v) (phospho Tyr118) antibody

Anti-Myosin Light Chain 2 (MLC-2v) (phospho Tyr118) antibody used in Western Blot (WB). GTX17511
Anti-Myosin Light Chain 2 (MLC-2v) (phospho Tyr118) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17511

Cat. No. GTX17511

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Product Note

This antibody detects Myosin light chain 2, slow skeletal/ventricular muscle isoform (MLC-2v) protein when phosphorylated at Ser15.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 118 (A-D-Y(p)-V-R) derived from human Myosin Light Chain 2 (MLC-2v).

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin light chain 2 , CMH10 , MLC-2s/v , MLC2

Background

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Myosin Light Chain 2 (MLC-2v) (phospho Tyr118) antibody used in Western Blot (WB). GTX17511

GTX17511 WB Image

WB analysis of extracts from U87 cells synchronized in interphase ( I ) and cytokinesis ( C ) using GTX17511 Myosin Light Chain 2 (MLC-2v) (phospho Tyr118) antibody.

Anti-Myosin Light Chain 2 (MLC-2v) (phospho Tyr118) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17511

GTX17511 ICC/IF Image

ICC/IF analysis of methanol-fixed U87 cells using GTX17511 Myosin Light Chain 2 (MLC-2v) (phospho Tyr118) antibody.

REFERENCE

There are currently no references for Myosin Light Chain 2 (MLC-2v) (phospho Tyr118) antibody (GTX17511). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Myosin Light Chain 2 (MLC-2v) (phospho Tyr118) antibody (GTX17511). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399