Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1,000 |
Not tested in other applications.
Calculated MW
Positive Control
chicken gizzard myosin II phosphorylated in vitro by PAK
Product Note
This antibody detects phosphorylated Ser19 Myosin Light Chain 2. The sequence around Ser19 is conserved among MYL9, MYL12A and MYL12B protein.
Predict Reactivity
Human(>80% identity)
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.3, 50% Glycerol, 1.0mg/ml BSA (IgG, Protease free)
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Chicken
Immunogen
synthetic phosphopeptide derived from the region of chicken MLC that contains serine 19 (serine 20 including the initiating methionine). Human (100% homologous) has not been tested, but is expected to cross-react.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
RRID
AB_380401
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
myosin light chain 9 , LC20 , MLC-2C , MLC2 , MRLC1 , MYRL2
Background
Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues. Phosphorylation of serine 19 (the major phosphorylation site and the preferred site for MLCK) activates myosin motor activity under physiological conditions.
Database
Research Area