APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 3-6 μg/ml |
| 2 μg/ml |
| 3-5 μg/10⁶ cells |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Synthetic Peptide: S(16) G Q E F D V P I G A V V K L C(31)
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
myosin VIIA , Hdb , Myo7 , USH1B , nmf371 , polka , sh-1 , sh1
Cellular Localization
Cytoplasm,Cytoplasm, cytoskeleton
Background
Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.
Database
Research Area
DATA IMAGES
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GTX23481 ICC/IF Image
ICC/IF analysis of L6 cells using GTX23481 Myosin VIIa antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
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GTX23481 WB Image
WB analysis of membrane-enriched extracts (30 μg lysate) of L6 using GTX23481 Myosin VIIa antibody.
Dilution : 5 μg/ml
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GTX23481 WB Image
WB analysis of mouse testes tissue extract using GTX23481 Myosin VIIa antibody.
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REFERENCE
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REVIEW
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