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Myotilin antibody

Anti-Myotilin antibody used in Western Blot (WB). GTX54355
Anti-Myotilin antibody used in IHC (Paraffin sections) (IHC-P). GTX54355
Anti-Myotilin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54355

Cat. No. GTX54355

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Myotilin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 259-314 of human MYOT (NP_001129412.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myotilin , LGMD1 , LGMD1A , MFM3 , TTID , TTOD

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.[provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-Myotilin antibody used in Western Blot (WB). GTX54355

GTX54355 WB Image

WB analysis of various sample lysates using GTX54355 Myotilin antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Myotilin antibody used in IHC (Paraffin sections) (IHC-P). GTX54355

GTX54355 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX54355 Myotilin antibody.
Dilution : 1:100

Anti-Myotilin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54355

GTX54355 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54355 Myotilin antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399