APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.01-0.03μg/ml |
| 2-3μg/ml |
|
Note :
IHC-P
Human Small Intestine shows staining of cytoplasm and membrane of enterocytes.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88676-PEP).
Predict Reactivity
Mouse, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NRAS proto-oncogene, GTPase , ALPS4 , CMNS , N-ras , NCMS , NRAS1 , NS6
Cellular Localization
Cell membrane , Golgi apparatus membrane
Background
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]
Database
Research Area
DATA IMAGES
|
GTX88676 IHC-P Image
IHC-P analysis of human small intestine using GTX88676 N-Ras antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
|
|
|
GTX88676 WB Image
WB analysis of MCF7 cell lysate (35μg protein in RIPA buffer) using GTX88676 N-RAS antibody.
Dilution : 0.5μg/ml
Dilution : 0.5μg/ml
|
REFERENCE
There are currently no references for N-RAS antibody (GTX88676). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for N-RAS antibody (GTX88676). Be the first to share your experience with this product.
