GeneTex
United States (US)

N-Ras antibody

GTX88676_847_IHC-P_18121411_372.jpg
GTX88676_2106_WB_18121411_105.jpg
GTX88676_2105_WB_18121411_741.jpg

Cat No. GTX88676

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.01-0.03μg/ml
IHC-P 2-3μg/ml

Note :

IHC-P
Human Small Intestine shows staining of cytoplasm and membrane of enterocytes.

Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88676-PEP).

Predict Reactivity

Mouse, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nras Proto-Oncogene, Gtpase,Alps4,Cmns,N-Ras,Ncms,Nras1,Ns6,Nras

Cellular Localization

Shuttles between the plasma membrane and the Golgi apparatus.

Background

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES
GTX88676_847_IHC-P_18121411_372.jpg

GTX88676 IHC-P Image

IHC-P analysis of human small intestine using GTX88676 N-Ras antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

GTX88676_2106_WB_18121411_105.jpg

GTX88676 WB Image

WB analysis of rat Intestines lysate using GTX88676 N-Ras antibody.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

GTX88676_2105_WB_18121411_741.jpg

GTX88676 WB Image

WB analysis of A431 lysate using GTX88676 N-Ras antibody.
Dilution : 0.01µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329