NAT8L antibody

Cat. No. GTX04238

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, FACS
Reactivity	Human, Mouse
Package	100 μg ($399)

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Datasheet

View product citations for antibody GTX04238 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.1-0.3μg/ml
ICC/IF	10μg/ml
FACS	10μg/ml

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris pH7.3, 0.5% BSA

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SVDSRFRGKGIAK, from the internal region of the protein sequence according to NP_001001985.3.

Purification

Ammonium sulphate precipitation followed by peptide affinity purification
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-acetyltransferase 8 like , CML3 , NACED , NAT8-LIKE

Cellular Localization

Cytoplasm , Membrane

Background

This gene encodes a single-pass membrane protein, which contains a conserved sequence of the GCN5 or NAT superfamily of N-acetyltransferases and is a member of the N-acyltransferase (NAT) superfamily. This protein is a neuron-specific protein and is the N-acetylaspartate (NAA) biosynthetic enzyme, catalyzing the NAA synthesis from L-aspartate and acetyl-CoA. NAA is a major storage and transport form of acetyl coenzyme A specific to the nervous system. The gene mutation results in primary NAA deficiency (hypoacetylaspartia). [provided by RefSeq, Dec 2010]

Database

Gene ID: 339983 NAT8L
UniProt: Q8N9F0 NAT8L

DATA IMAGES

GTX04238 FACS Image

FACS analysis of PFA-fixed Kelly cells using GTX04238 NAT8L antibody.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

GTX04238 ICC/IF Image

ICC/IF analysis of PFA-fixed HEK293 cells using GTX04238 NAT8L antibody.
Negative control : Unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

GTX04238 WB Image

WB analysis of mouse fetal brain, adult brain, and human brain tissue lysates using GTX04238 NAT8L antibody preincubated with (Lane B, D, F) or without (Lane A, C, E) immunogen peptide.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 399

Qty