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NCF1 antibody

Anti-NCF1 antibody used in Western Blot (WB). GTX102957
Anti-NCF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102957
Anti-NCF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102957

Cat. No. GTX102957

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NCF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NCF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176911

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neutrophil cytosolic factor 1 , NCF1A , NOXO2 , SH3PXD1A , p47phox

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NCF1 antibody used in Western Blot (WB). GTX102957

GTX102957 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX102957 diluted at 1:5000

Anti-NCF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102957

GTX102957 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using NCF1(GTX102957) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NCF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102957

GTX102957 ICC/IF Image

NCF1 antibody detects NCF1 protein at cytoplasm by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NCF1 protein stained by NCF1 antibody (GTX102957) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169