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NCF1 antibody

Anti-NCF1 antibody used in Western Blot (WB). GTX20795

Cat. No. GTX20795

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine, Dolphin
Package
50 μg ($399)
See all NCF1 products

APPLICATION

Application Note

Western Blot - Approx 47kDa band seen in U937 cell lysates. Recommended for use at 0.2-2μg/ml.

Calculated MW

45 kDa. ( Note )

Positive Control

U937 cell lysates

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: CSESTKRKLASAV.

Purification

IgG fraction
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neutrophil cytosolic factor 1 , NCF1A , NOXO2 , SH3PXD1A , p47phox

Cellular Localization

Cytoplasm, cytosol , Membrane

Background

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NCF1 antibody used in Western Blot (WB). GTX20795

GTX20795 WB Image

GTX20795 staining (0.2g/ml) of U937 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399