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NCKAP1 antibody [C1C2], Internal

Anti-NCKAP1 antibody [C1C2], Internal used in IHC (Frozen sections) (IHC-Fr). GTX105682
Anti-NCKAP1 antibody [C1C2], Internal used in Western Blot (WB). GTX105682
Anti-NCKAP1 antibody [C1C2], Internal used in Western Blot (WB). GTX105682
Anti-NCKAP1 antibody [C1C2], Internal used in Western Blot (WB). GTX105682

Cat. No. GTX105682

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NCKAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

129 kDa. ( Note )

Positive Control

Mouse brain , rat brain , NCKAP1-transfected 293T

Predict Reactivity

Zebrafish, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NCKAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950977

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NCK associated protein 1 , HEM2 , NAP1 , NAP125 , p125Nap1

Cellular Localization

Cell membrane; Single-pass membrane protein; Cytoplasmic side , Cell projection , lamellipodium membrane; Single-pass membrane protein; Cytoplasmic side

Background

Is part of lamellipodial complex that controls Rac-dependent actin remodeling.

Database

Research Area

DATA IMAGES

Anti-NCKAP1 antibody [C1C2], Internal used in IHC (Frozen sections) (IHC-Fr). GTX105682

GTX105682 IHC-Fr Image

NCKAP1 antibody [C1C2], Internal detects NCKAP1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: NCKAP1 stained by NCKAP1 antibody [C1C2], Internal (GTX105682) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NCKAP1 antibody [C1C2], Internal used in Western Blot (WB). GTX105682

GTX105682 WB Image

NCKAP1 antibody [C1C2], Internal detects NCKAP1 protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
5 % SDS-PAGE
NCKAP1 antibody [C1C2], Internal (GTX105682) dilution: 1:1000

Anti-NCKAP1 antibody [C1C2], Internal used in Western Blot (WB). GTX105682

GTX105682 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX105682 diluted at 1:1000

Anti-NCKAP1 antibody [C1C2], Internal used in Western Blot (WB). GTX105682

GTX105682 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [C1C2], Internal (GTX105682) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169